Hotspot

trnV-GAC-psbJ

Genome · 79093-79825 · 733 bp

Back to Adenophora

Pi 0.0242

MarkerSeek score 63.00

Primer available yes

Label trnV-GAC-psbJ

Feature Metadata

label_name: trnV-GAC-psbJ
feature_type: igs
region: Genome
length_bp: 733
variable_sites: 72
pi: 0.0242
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-psbJ_p1	AGCCTTGTTCGTCTTTGGTT	GGCGCAGGTCTTCTAAATCA	45.0	50.0	57.9	58.0	1425-1585 bp	0.9	84.8
trnV-GAC-psbJ_p2	TCGACTGATTCAGGGATCGA	GGCGCAGGTCTTCTAAATCA	50.0	50.0	57.9	58.0	1681-1855 bp	0.9	84.4
trnV-GAC-psbJ_p3	TCAGTTCTATTGGGGGCAGA	GGCGCAGGTCTTCTAAATCA	50.0	50.0	58.0	58.0	1158-1167 bp	0.8	73.4
trnV-GAC-psbJ_p4	GTCAGTTCTATTGGGGGCAG	GGCGCAGGTCTTCTAAATCA	55.0	50.0	58.0	58.0	1159-1168 bp	0.8	73.3
trnV-GAC-psbJ_p5	TCAGTTCTATTGGGGGCAGA	AGTGATTCCGTCGTGATTCG	50.0	50.0	58.0	58.1	1248-1257 bp	0.8	72.7