Hotspot

trnC-GCA-petN

Genome · 62866-64957 · 2092 bp

Back to Adenophora

Pi 0.0963

MarkerSeek score 80.90

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: Genome
length_bp: 2092
variable_sites: 551
pi: 0.0963
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTTCCCCAGTTCAAGCCTG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.3	58.2	1755-2741 bp	0.8	80.4
trnC-GCA-petN_p2	TTTTTCCCCAGTTCAAGCCT	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.5	58.2	1756-2742 bp	0.8	79.0
trnC-GCA-petN_p3	TTTTCCCCAGTTCAAGCCTG	AGAGACCACTTCTTCCCCAT	50.0	50.0	58.3	57.6	1816-2802 bp	0.8	78.8
trnC-GCA-petN_p4	TTTTTCCCCAGTTCAAGCCT	AGAGACCACTTCTTCCCCAT	45.0	50.0	57.5	57.6	1817-2803 bp	0.8	77.4
trnC-GCA-petN_p5	CGGGGGATTGCAAATCCTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.8	58.2	1773-2759 bp	0.8	76.3