Hotspot

psbJ-ndhC

Genome · 37812-40633 · 2822 bp

Back to Adenophora

Pi 0.0639

MarkerSeek score 77.40

Primer available yes

Label psbJ-ndhC

Feature Metadata

label_name: psbJ-ndhC
feature_type: igs
region: Genome
length_bp: 2822
variable_sites: 485
pi: 0.0639
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-ndhC_p1	ATTCCGGATTGGGTTCATCC	GGGGCTAATACTCCCGAAAG	50.0	55.0	57.6	57.5	1412-2946 bp	0.3	56.7
psbJ-ndhC_p2	ATTCCGGATTGGGTTCATCC	GGGGGCTAATACTCCCGAAA	50.0	55.0	57.6	58.9	1413-2947 bp	0.3	54.2
psbJ-ndhC_p3	ATTCCGGATTGGGTTCATCC	AATGGGGGCTAATACTCCCG	50.0	55.0	57.6	58.9	1416-2950 bp	0.3	53.6
psbJ-ndhC_p4	ATTCCGGATTGGGTTCATCC	GGGGGCTAATACTCCCGAA	50.0	57.9	57.6	58.2	1413-2947 bp	0.3	51.8
psbJ-ndhC_p5	ATTCCGGATTGGGTTCATCC	ATGGGGGCTAATACTCCCG	50.0	57.9	57.6	58.2	1415-2949 bp	0.3	51.3