Hotspot

psbT-psbB

Genome · 27992-28219 · 228 bp

Back to Adenophora

Pi 0.0196

MarkerSeek score 67.20

Primer available yes

Label psbT-psbB

Feature Metadata

label_name: psbT-psbB
feature_type: igs
region: Genome
length_bp: 228
variable_sites: 14
pi: 0.0196
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbB_p1	GAACTTTTGGCGGCTCTCTA	GCTCAAGTCGAATTTGGAGC	50.0	50.0	57.9	57.5	360-383 bp	1.0	82.1
psbT-psbB_p2	GAACTTTTGGCGGCTCTCTA	GGTGCTAGAACCCTCTTCAG	50.0	55.0	57.9	57.3	414-437 bp	1.0	80.6
psbT-psbB_p3	AACTTTTGGCGGCTCTCTAA	GCTCAAGTCGAATTTGGAGC	45.0	50.0	57.4	57.5	359-382 bp	1.0	78.4
psbT-psbB_p4	ACTTTTGGCGGCTCTCTAAA	GCTCAAGTCGAATTTGGAGC	45.0	50.0	57.4	57.5	358-381 bp	1.0	78.4
psbT-psbB_p5	ACTTTTGGCGGCTCTCTAAA	GGTGCTAGAACCCTCTTCAG	45.0	55.0	57.4	57.3	412-435 bp	1.0	76.8