Hotspot

rpoA-petD

Genome · 23200-24383 · 1184 bp

Back to Adenophora

Pi 0.0438

MarkerSeek score 73.20

Primer available yes

Label rpoA-petD

Feature Metadata

label_name: rpoA-petD
feature_type: igs
region: Genome
length_bp: 1184
variable_sites: 159
pi: 0.0438
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-petD_p1	AGTTCGGGATTTATTTACCGCA	CCCATTTCGTCGTCCAGTAG	40.9	55.0	58.1	58.1	1334-1349 bp	0.9	67.1
rpoA-petD_p2	AGTTCGGGATTTATTTACCGCA	TTGGTTGGGTATTGGTGCAA	40.9	45.0	58.1	57.9	1277-1292 bp	0.9	67.1
rpoA-petD_p3	AGTTCGGGATTTATTTACCGCA	TTTGGTTGGGTATTGGTGCA	40.9	45.0	58.1	57.9	1278-1293 bp	0.9	67.1
rpoA-petD_p4	AGTTCGGGATTTATTTACCGCA	CAGTAGCGACAACCGTCTTT	40.9	50.0	58.1	58.2	1320-1335 bp	0.9	66.0
rpoA-petD_p5	GTTCGGGATTTATTTACCGCA	CCCATTTCGTCGTCCAGTAG	42.9	55.0	56.7	58.1	1333-1348 bp	0.9	65.6