Hotspot

rrn16

IRb · 100298-101788 · 1491 bp

Back to Adenocalymma

Pi 0.0615

MarkerSeek score 79.50

Primer available yes

Label rrn16

Feature Metadata

label_name: rrn16
feature_type: rRNA
region: IRb
length_bp: 1491
variable_sites: 535
pi: 0.0615
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16_p1	TCGAATCCGCTTTGTCTACG	AACTCAGACGTAGCTCCCTT	50.0	50.0	58.1	58.1	1621-1627 bp	0.916667	76.6
rrn16_p2	CGAATCCGCTTTGTCTACGA	AACTCAGACGTAGCTCCCTT	50.0	50.0	58.1	58.1	1620-1626 bp	0.916667	76.5
rrn16_p3	TCGAATCCGCTTTGTCTACG	TGTGAAGCAGTGTCAAACCA	50.0	45.0	58.1	57.9	1593-1594 bp	0.916667	75.9
rrn16_p4	CGAATCCGCTTTGTCTACGA	TGTGAAGCAGTGTCAAACCA	50.0	45.0	58.1	57.9	1592-1593 bp	0.916667	75.8
rrn16_p5	CGAATCCGCTTTGTCTACGA	TTTTGGGTGTGAAGCAGTGT	50.0	45.0	58.1	58.2	1599 bp	0.833333	72.4