Hotspot

rpoA

LSC · 78157-79209 · 1053 bp

Back to Adenocalymma

Pi 0.0683

MarkerSeek score 84.20

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1053
variable_sites: 206
pi: 0.0683
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	ACGTGTGTATCTAGGGAGGG	CCGAGAACTGGTTCACGTAG	55.0	55.0	57.9	58.0	1678-1784 bp	1.0	91.3
rpoA_p2	ACGTGTGTATCTAGGGAGGG	CGAGAACTGGTTCACGTAGG	55.0	55.0	57.9	58.0	1677-1783 bp	1.0	91.3
rpoA_p3	ACGTGTGTATCTAGGGAGGG	GGAAGAGACGCAGCATTACA	55.0	50.0	57.9	58.0	1348-1430 bp	0.916667	88.0
rpoA_p4	ACGTGTGTATCTAGGGAGGG	CGCTCAAACTGCAACAGAAG	55.0	50.0	57.9	58.0	1449-1518 bp	0.916667	87.9
rpoA_p5	ACGTGTGTATCTAGGGAGGG	AGGGTACGAGAAAGGGAACA	55.0	50.0	57.9	58.0	1478-1546 bp	0.583333	74.6