Hotspot

accD

LSC · 57797-59521 · 1725 bp

Back to Adenocalymma

Pi 0.164

MarkerSeek score 79.50

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1725
variable_sites: 929
pi: 0.164
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	CGATGCGAAGACAGAGGAAA	CAGGCATAAGCGCTTTTTCC	50.0	50.0	58.0	58.1	2623-2982 bp	0.666667	77.9
accD_p2	CGATGCGAAGACAGAGGAAA	GCGCCCCTGATCTATTTGAA	50.0	50.0	58.0	58.0	2680-2972 bp	0.333333	65.0
accD_p3	TGTTCGATGCGAAGACAGAG	GCGCCCCTGATCTATTTGAA	50.0	50.0	58.0	58.0	2684-2961 bp	0.25	61.6
accD_p4	CGATGCGAAGACAGAGGAAA	AAGAAACCGTAAAGGCCCAG	50.0	50.0	58.0	58.1	2984 bp	0.083333	42.5
accD_p5	CGATGCGAAGACAGAGGAAA	GAAACCGTAAAGGCCCAGAA	50.0	50.0	58.0	58.1	2982 bp	0.083333	42.5