Hotspot

ycf1-trnN-GUU

IRa · 134472-135634 · 1163 bp

Back to Adenocalymma

Pi 0.225

MarkerSeek score 75.40

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: IRa
length_bp: 1163
variable_sites: 516
pi: 0.225
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.71
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	ATGAACACCATGAGCTGTCC	GTGTGGGTCTTCAGCGTATT	50.0	50.0	57.9	57.9	1203-2395 bp	0.833333	83.6
ycf1-trnN-GUU_p2	ATGAACACCATGAGCTGTCC	TTAACGGTCAAAGTGAGCCC	50.0	50.0	57.9	58.1	858-2050 bp	0.833333	83.5
ycf1-trnN-GUU_p3	ATGAACACCATGAGCTGTCC	GGTAAACGCGCTTTCACATC	50.0	50.0	57.9	58.1	1464-2657 bp	0.833333	83.4
ycf1-trnN-GUU_p4	ATGAACACCATGAGCTGTCC	TCTTAACCCGAAATGGCTGG	50.0	50.0	57.9	57.9	1443-2636 bp	0.833333	83.4
ycf1-trnN-GUU_p5	ATGAACACCATGAGCTGTCC	TGAACTCTCGGGAGAATCGA	50.0	50.0	57.9	57.9	1058-2250 bp	0.833333	83.4