Hotspot

ndhD-psaC

SSC · 122364-122495 · 132 bp

Back to Adenocalymma

Pi 0.0444

MarkerSeek score 81.50

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 132
variable_sites: 21
pi: 0.0444
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	GAACCCGCAAATATTGGCAA	TGTGAATCTGCCTGTCCAAC	45.0	50.0	57.3	58.1	263-276 bp	1.0	84.9
ndhD-psaC_p2	AGGAACCCGCAAATATTGGC	TGTGAATCTGCCTGTCCAAC	50.0	50.0	58.9	58.1	265-278 bp	1.0	83.4
ndhD-psaC_p3	GAACCCGCAAATATTGGCAA	GATGTGAATCTGCCTGTCCA	45.0	50.0	57.3	57.6	265-278 bp	1.0	82.4
ndhD-psaC_p4	CCCGCAAATATTGGCAAAACT	TGTGAATCTGCCTGTCCAAC	42.9	50.0	58.0	58.1	260-273 bp	1.0	82.1
ndhD-psaC_p5	GAACCCGCAAATATTGGCAA	GCCTGTCCAACGGATTTCTT	45.0	50.0	57.3	58.5	254-267 bp	0.916667	78.6