Hotspot

trnN-GUU-ycf1

IRb · 108444-109606 · 1163 bp

Back to Adenocalymma

Pi 0.227

MarkerSeek score 75.60

Primer available yes

Label trnN-GUU-ycf1

Feature Metadata

label_name: trnN-GUU-ycf1
feature_type: igs
region: IRb
length_bp: 1163
variable_sites: 580
pi: 0.227
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.72
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ycf1_p1	GTGTGGGTCTTCAGCGTATT	ATGAACACCATGAGCTGTCC	50.0	50.0	57.9	57.9	1203-2395 bp	0.75	80.3
trnN-GUU-ycf1_p2	TTAACGGTCAAAGTGAGCCC	ATGAACACCATGAGCTGTCC	50.0	50.0	58.1	57.9	858-2050 bp	0.75	80.1
trnN-GUU-ycf1_p3	GGTAAACGCGCTTTCACATC	ATGAACACCATGAGCTGTCC	50.0	50.0	58.1	57.9	1464-2657 bp	0.75	80.1
trnN-GUU-ycf1_p4	TCTTAACCCGAAATGGCTGG	ATGAACACCATGAGCTGTCC	50.0	50.0	57.9	57.9	1443-2636 bp	0.75	80.1
trnN-GUU-ycf1_p5	TGAACTCTCGGGAGAATCGA	ATGAACACCATGAGCTGTCC	50.0	50.0	57.9	57.9	1058-2250 bp	0.75	80.0