Hotspot

trnR-ACG-trnN-GUU

IRb · 107885-108370 · 486 bp

Back to Adenocalymma

Pi 0.0541

MarkerSeek score 83.10

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 486
variable_sites: 135
pi: 0.0541
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	GGGGTTCGAATCCCTCCT	CCGTTGTTCCTCAGTAGCTC	61.1	55.0	57.9	58.0	590 bp	0.916667	65.1
trnR-ACG-trnN-GUU_p2	GGGGTTCGAATCCCTCCT	TGGTCGTAGGTTCGAATCCT	61.1	50.0	57.9	58.2	541 bp	0.916667	64.3
trnR-ACG-trnN-GUU_p3	GGGGTTCGAATCCCTCCT	CGATTGGTCGTAGGTTCGAA	61.1	50.0	57.9	57.7	545 bp	0.916667	63.4
trnR-ACG-trnN-GUU_p4	GGGGTTCGAATCCCTCCTC	CCGTTGTTCCTCAGTAGCTC	63.2	55.0	59.2	58.0	590 bp	0.833333	58.9
trnR-ACG-trnN-GUU_p5	GGGGTTCGAATCCCTCCTC	TGGTCGTAGGTTCGAATCCT	63.2	50.0	59.2	58.2	541 bp	0.833333	58.0