Hotspot

ycf4-cemA

LSC · 60657-61536 · 880 bp

Back to Adenocalymma

Pi 0.0569

MarkerSeek score 84.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 880
variable_sites: 244
pi: 0.0569
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TCGTGATGCTTTTCTACGGG	GGAATTCCTGAGCCCAAGAG	50.0	55.0	58.0	58.0	1609-1700 bp	0.833333	84.2
ycf4-cemA_p2	CGTGATGCTTTTCTACGGGA	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.0	58.1	1277-1348 bp	0.75	80.0
ycf4-cemA_p3	CGTGATGCTTTTCTACGGGA	GGAATTCCTGAGCCCAAGAG	50.0	55.0	58.0	58.0	1628-1699 bp	0.75	78.7
ycf4-cemA_p4	TTCTTCTGGTGACCCGTACT	GGAATTCCTGAGCCCAAGAG	50.0	55.0	58.0	58.0	1395-1427 bp	0.416667	59.9
ycf4-cemA_p5	TAGTGGTCGGAACTTCCAGT	GGAATTCCTGAGCCCAAGAG	50.0	55.0	58.0	58.0	1753-1763 bp	0.166667	46.6