Hotspot

ccsA-ndhD

SSC · 120214-120510 · 297 bp

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Pi 0.0232

MarkerSeek score 47.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 297
variable_sites: 15
pi: 0.0232
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GTAAATTCCGCACTTGTGGC	GGGCATGAGTGGTTTTGTTG	50.0	50.0	58.0	57.8	752-759 bp	1.0	81.1
ccsA-ndhD_p2	TTGGACCGTATTCGCGATTT	GGGCATGAGTGGTTTTGTTG	45.0	50.0	58.0	57.8	807-814 bp	1.0	81.0
ccsA-ndhD_p3	GTAAATTCCGCACTTGTGGC	TTATCTCGACGAAATGGGCG	50.0	50.0	58.0	58.2	842-849 bp	1.0	80.9
ccsA-ndhD_p4	TTGGACCGTATTCGCGATTT	TTATCTCGACGAAATGGGCG	45.0	50.0	58.0	58.2	897-904 bp	1.0	80.8
ccsA-ndhD_p5	TTGGACCGTATTCGCGATTT	GCATGAGTGGTTTTGTTGCA	45.0	45.0	58.0	57.8	805-812 bp	1.0	80.5