Hotspot

ndhF-rpl32

SSC · 116799-118028 · 1230 bp

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Pi 0.00557
MarkerSeek score 53.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1230
variable_sites
18
pi
0.00557
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGGAAGTGGCACAAAAGGTA GGTTGAAAGCAAGGGAAGGA 50.0 50.0 58.0 58.0 1389-1813 bp 1.0 85.4
ndhF-rpl32_p2 GCCCACATACGACGAAGATT GGTTGAAAGCAAGGGAAGGA 50.0 50.0 58.1 58.0 1460-1884 bp 1.0 84.9
ndhF-rpl32_p3 GGGAAGTGGCACAAAAGGTA TGGGCGATGGGGAAAATAAG 50.0 50.0 58.0 57.9 1151-1568 bp 1.0 84.7
ndhF-rpl32_p4 GCCCACATACGACGAAGATT TGGGCGATGGGGAAAATAAG 50.0 50.0 58.1 57.9 1222-1639 bp 1.0 84.2
ndhF-rpl32_p5 GGGAAGTGGCACAAAAGGTA AAATGGGCGATGGGGAAAAT 50.0 45.0 58.0 58.1 1154-1571 bp 1.0 84.2