Hotspot

rps19-rpl2

IRb · 88973-89062 · 90 bp

Back to Adansonia

Pi 0.0133

MarkerSeek score 56.00

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 90
variable_sites: 3
pi: 0.0133
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TGGTAGATGCTCTGGACCAA	TTGATTCTTCGTCGCCGTAG	50.0	50.0	58.0	58.1	225-241 bp	1.0	83.9
rps19-rpl2_p2	ATGGTAGATGCTCTGGACCA	TTGATTCTTCGTCGCCGTAG	50.0	50.0	57.8	58.1	226-242 bp	1.0	82.8
rps19-rpl2_p3	TGGTAGATGCTCTGGACCAA	TTGGGGTTATCCTGCACTTG	50.0	50.0	58.0	57.8	283-299 bp	1.0	82.7
rps19-rpl2_p4	TGGTAGATGCTCTGGACCAA	ATTTGATTCTTCGTCGCCGT	50.0	45.0	58.0	58.3	227-243 bp	1.0	82.4
rps19-rpl2_p5	ATGGTAGATGCTCTGGACCA	TTGGGGTTATCCTGCACTTG	50.0	50.0	57.8	57.8	284-300 bp	1.0	81.7