Hotspot

ycf4-cemA

LSC · 64280-65263 · 984 bp

Back to Adansonia

Pi 0.00327

MarkerSeek score 59.90

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 984
variable_sites: 10
pi: 0.00327
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GCGTGTACCGATTGAAGTCT	GAGATCCACCAGGGCAAAAA	50.0	50.0	58.0	58.1	1075-1093 bp	1.0	81.6
ycf4-cemA_p2	TGACTCCGCGAGAAATTGAG	GAGATCCACCAGGGCAAAAA	50.0	50.0	58.0	58.1	1124-1142 bp	1.0	81.5
ycf4-cemA_p3	TGAGAATTTGACTCCGCGAG	GAGATCCACCAGGGCAAAAA	50.0	50.0	58.0	58.1	1132-1150 bp	1.0	81.5
ycf4-cemA_p4	CGTTCCCTTGACTCGTACTG	GAGATCCACCAGGGCAAAAA	55.0	50.0	58.0	58.1	1153-1171 bp	1.0	81.3
ycf4-cemA_p5	TTGCGTGTACCGATTGAAGT	GAGATCCACCAGGGCAAAAA	45.0	50.0	57.8	58.1	1077-1095 bp	1.0	80.4