Hotspot

ndhC-trnV-UAC

LSC · 53493-54718 · 1226 bp

Back to Adansonia

Pi 0.00805

MarkerSeek score 58.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1226
variable_sites: 26
pi: 0.00805
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	ACCCTTTCTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	1341-1391 bp	1.0	82.4
ndhC-trnV-UAC_p2	CGGGGCTAAAACTCCAGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	1329-1379 bp	1.0	82.4
ndhC-trnV-UAC_p3	ATCGGGGCTAAAACTCCAGA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.4	57.8	1331-1381 bp	1.0	80.5
ndhC-trnV-UAC_p4	AATCGGGGCTAAAACTCCAG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.6	57.8	1332-1382 bp	1.0	80.5
ndhC-trnV-UAC_p5	ACCCTTTCTAATCGGGGCTA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.8	57.5	1340-1390 bp	1.0	79.6