Hotspot

ycf3-trnS-GGA

LSC · 46476-47442 · 967 bp

Back to Adansonia

Pi 0.00397

MarkerSeek score 59.80

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 967
variable_sites: 11
pi: 0.00397
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	CATTTATCCGCGATCTAGGCA	TGGCGAATTAGGTGAAGGTG	47.6	50.0	58.0	57.9	1089-1100 bp	1.0	73.4
ycf3-trnS-GGA_p2	CATTTATCCGCGATCTAGGCA	TTAGGTGAAGGTGCGGAAAG	47.6	50.0	58.0	57.8	1082-1093 bp	1.0	72.9
ycf3-trnS-GGA_p3	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTGAA	47.6	45.0	58.0	57.8	1020-1031 bp	1.0	72.9
ycf3-trnS-GGA_p4	CATTTATCCGCGATCTAGGCA	GTGCGGAAAGAGAGGGATTC	47.6	55.0	58.0	58.3	1072-1083 bp	1.0	71.6
ycf3-trnS-GGA_p5	CATTTATCCGCGATCTAGGCA	GAATTAGGTGAAGGTGCGGA	47.6	50.0	58.0	57.6	1085-1096 bp	1.0	71.1