Hotspot

psbZ-trnG-UCC

LSC · 37385-38231 · 847 bp

Back to Adansonia

Pi 0.0131

MarkerSeek score 64.60

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 847
variable_sites: 31
pi: 0.0131
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	TTGCTTCTCCTGATGGTTGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	827-1004 bp	1.0	83.6
psbZ-trnG-UCC_p2	GCTTCTCCTGATGGTTGGTT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.8	57.8	825-1002 bp	1.0	83.6
psbZ-trnG-UCC_p3	TTGCTTCTCCTGATGGTTGG	TACATTGTCCTGGGCGGATA	50.0	50.0	57.8	58.2	863-1040 bp	1.0	83.2
psbZ-trnG-UCC_p4	GCTTCTCCTGATGGTTGGTT	TACATTGTCCTGGGCGGATA	50.0	50.0	57.8	58.2	861-1038 bp	1.0	83.2
psbZ-trnG-UCC_p5	TTGCTTCTCCTGATGGTTGG	AAATACATTGTCCTGGGCGG	50.0	50.0	57.8	58.2	866-1043 bp	1.0	83.0