Hotspot

ycf2

LSC · 90056-96892 · 6837 bp

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Pi 0.00407

MarkerSeek score 55.40

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: LSC
length_bp: 6837
variable_sites: 51
pi: 0.00407
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TGAATCATCCCCATCGCTTC	TGCCAGATACCTGTGACTCA	50.0	50.0	57.7	58.1	213 bp	1.0	79.7
ycf2_p2	TCTTGAATCATCCCCATCGC	TGCCAGATACCTGTGACTCA	50.0	50.0	57.7	58.1	216 bp	1.0	79.6
ycf2_p3	AATCATCCCCATCGCTTCTG	TGCCAGATACCTGTGACTCA	50.0	50.0	57.7	58.1	211 bp	1.0	79.6
ycf2_p4	CTTGAATCATCCCCATCGCT	TGCCAGATACCTGTGACTCA	50.0	50.0	57.7	58.1	215 bp	1.0	79.6
ycf2_p5	GAATCATCCCCATCGCTTCT	TGCCAGATACCTGTGACTCA	50.0	50.0	57.4	58.1	212 bp	1.0	77.3