Hotspot

rpl32-trnL-UAG

SSC · 117343-118745 · 1403 bp

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Pi 0.0133

MarkerSeek score 56.90

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1403
variable_sites: 34
pi: 0.0133
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ACAAACAAGTAATAAAGCCTTGGA	AGCAGCGTGTCTACCAATTT	33.3	45.0	57.0	57.8	1447-1463 bp	1.0	43.4
rpl32-trnL-UAG_p2	ACAAACAAGTAATAAAGCCTTGGA	GAGCAGCGTGTCTACCAATT	33.3	50.0	57.0	58.3	1448-1464 bp	1.0	43.4
rpl32-trnL-UAG_p3	ACAAACAAGTAATAAAGCCTTGGA	TCCTAAGAGCAGCGTGTCTA	33.3	50.0	57.0	57.9	1454-1470 bp	1.0	43.3
rpl32-trnL-UAG_p4	ACAAACAAGTAATAAAGCCTTGGA	CCTAAGAGCAGCGTGTCTAC	33.3	55.0	57.0	57.8	1453-1469 bp	1.0	43.3
rpl32-trnL-UAG_p5	ACAAACAAGTAATAAAGCCTTGGA	CTAAGAGCAGCGTGTCTACC	33.3	55.0	57.0	57.8	1452-1468 bp	1.0	43.3