Hotspot

trnF-GAA-ndhJ

LSC · 50429-50995 · 567 bp

Back to Actinodaphne

Pi 0.00676

MarkerSeek score 60.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 567
variable_sites: 7
pi: 0.00676
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	ATCCACGCCTGAAACGTATC	55.0	50.0	58.0	58.1	723-741 bp	1.0	82.2
trnF-GAA-ndhJ_p2	TCCTCTAATAGGGCGATGCA	ATCCACGCCTGAAACGTATC	50.0	50.0	58.0	58.1	755-773 bp	1.0	82.0
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	687-705 bp	1.0	81.9
trnF-GAA-ndhJ_p4	CTGGTAGAGCAGAGGACTGA	ATCCACGCCTGAAACGTATC	55.0	50.0	57.9	58.1	711-729 bp	1.0	81.2
trnF-GAA-ndhJ_p5	TGACATAGACCCCAGTCCTC	ATCCACGCCTGAAACGTATC	55.0	50.0	57.8	58.1	770-788 bp	1.0	80.8