Hotspot

trnT-GGU-psbD

LSC · 33739-35179 · 1441 bp

Back to Actinodaphne

Pi 0.00833

MarkerSeek score 63.20

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1441
variable_sites: 23
pi: 0.00833
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTCCTTAACC	55.0	55.0	58.1	57.9	1583-1588 bp	1.0	80.0
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTCCTTAACC	50.0	55.0	58.1	57.9	1586-1591 bp	1.0	79.8
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1591-1596 bp	1.0	78.8
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1594-1599 bp	1.0	78.6
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	CGAAACGGTCCCTCCTTAAC	55.0	55.0	58.1	58.3	1584-1589 bp	1.0	78.5