Hotspot

accD

LSC · 59440-60273 · 834 bp

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Pi 0.0148

MarkerSeek score 62.70

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 834
variable_sites: 70
pi: 0.0148
unique_haplotype_count: 38
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TTCAGGCACTTGGGATCCTA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1475-1942 bp	1.0	91.2
accD_p2	TCAGGCACTTGGGATCCTAT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	1474-1941 bp	1.0	90.0
accD_p3	GGGGCATTCTAAAGACCTCG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	1456-1771 bp	0.622222	73.7
accD_p4	TTTCGAAGAGGCGCCTTATC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	1519-1619 bp	0.466667	66.1
accD_p5	TCCAGATCCAGAGCCTTCTC	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.2	58.0	1501-1601 bp	0.488889	65.9