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rps12-psbB

LSC · 71857-74867 · 3011 bp

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Pi 0.0379

MarkerSeek score 68.60

Primer available yes

Label rps12-psbB

Feature Metadata

label_name: rps12-psbB
feature_type: igs
region: LSC
length_bp: 3011
variable_sites: 410
pi: 0.0379
unique_haplotype_count: 41
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-psbB_p1	TCATGCCTTCTGCCAACTTT	CAGAGAGAAGAAGGCCTCCT	45.0	55.0	58.0	58.1	643-1218 bp	0.2	58.3
rps12-psbB_p2	TTCATGCCTTCTGCCAACTT	CAGAGAGAAGAAGGCCTCCT	45.0	55.0	58.0	58.1	644-1219 bp	0.2	58.3
rps12-psbB_p3	TTTCATGCCTTCTGCCAACT	CAGAGAGAAGAAGGCCTCCT	45.0	55.0	58.0	58.1	645-1220 bp	0.2	58.3
rps12-psbB_p4	GGCCTTGGTGGATGATAAGG	CAGAGAGAAGAAGGCCTCCT	55.0	55.0	58.0	58.1	103-667 bp	0.111111	55.3
rps12-psbB_p5	TCATGCCTTCTGCCAACTTT	TTCTACCGAACCAGATCCGT	45.0	50.0	58.0	58.2	1148-1199 bp	0.044444	40.5