Hotspot

atpB-rbcL

LSC · 55599-56361 · 763 bp

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Pi 0.00541

MarkerSeek score 65.20

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 763
variable_sites: 39
pi: 0.00541
unique_haplotype_count: 32
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GATTTGAACGATACGCCCCT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.1	58.0	862-917 bp	1.0	79.3
atpB-rbcL_p2	GATTTGAACGATACGCCCCT	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.1	58.1	861-916 bp	1.0	79.1
atpB-rbcL_p3	TGCATCTAGTACGGGACCAA	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.1	58.0	883-938 bp	1.0	78.5
atpB-rbcL_p4	GAACGATACGCCCCTGATTT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.1	58.0	885 bp	0.044444	33.2
atpB-rbcL_p5	GAACGATACGCCCCTGATTT	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.1	58.1	884 bp	0.044444	33.0