Hotspot

trnF (GAA)-ndhJ

LSC · 49011-49719 · 709 bp

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Pi 0.0143
MarkerSeek score 64.50
Primer available yes
Label trnF (GAA)-ndhJ

Feature Metadata

label_name
trnF (GAA)-ndhJ
feature_type
igs
region
LSC
length_bp
709
variable_sites
86
pi
0.0143
unique_haplotype_count
34
primer_available
yes
alignment_reliability
0.98
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF (GAA)-ndhJ_p1 CCTCGTGTCACCAGTTCAAA CGTAAGGATTATATTGCCCCCA 50.0 45.5 58.1 57.7 782-809 bp 1.0 73.2
trnF (GAA)-ndhJ_p2 CCTCGTGTCACCAGTTCAAA ACGTAAGGATTATATTGCCCCC 50.0 45.5 58.1 57.7 783-810 bp 1.0 73.2
trnF (GAA)-ndhJ_p3 AATCCTCGTGTCACCAGTTC CGTAAGGATTATATTGCCCCCA 50.0 45.5 57.5 57.7 785-812 bp 1.0 70.0
trnF (GAA)-ndhJ_p4 AATCCTCGTGTCACCAGTTC ACGTAAGGATTATATTGCCCCC 50.0 45.5 57.5 57.7 786-813 bp 1.0 70.0
trnF (GAA)-ndhJ_p5 TGGTAGAGCAGAGGACTGAA CGTAAGGATTATATTGCCCCCA 50.0 45.5 57.4 57.7 805-832 bp 1.0 68.8