Hotspot

trnT (UGU)-trnL (UAA)

LSC · 47351-47978 · 628 bp

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Pi 0.0193

MarkerSeek score 70.90

Primer available yes

Label trnT (UGU)-trnL (UAA)

Feature Metadata

label_name: trnT (UGU)-trnL (UAA)
feature_type: igs
region: LSC
length_bp: 628
variable_sites: 80
pi: 0.0193
unique_haplotype_count: 33
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT (UGU)-trnL (UAA)_p1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	55.0	50.0	57.9	58.0	645-729 bp	1.0	90.9
trnT (UGU)-trnL (UAA)_p2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	55.0	50.0	57.9	58.1	653-737 bp	1.0	90.4
trnT (UGU)-trnL (UAA)_p3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	50.0	50.0	57.8	58.0	677-761 bp	1.0	90.0
trnT (UGU)-trnL (UAA)_p4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.8	58.1	685-769 bp	1.0	89.5
trnT (UGU)-trnL (UAA)_p5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	659-743 bp	1.0	89.0