Hotspot

ycf1

SSC · 128546-134146 · 5601 bp

Pi 0.0141

MarkerSeek score 49.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5601
variable_sites: 242
pi: 0.0141
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGATTCTTTTGGTTTTGTGCA	TCATACCAATCAATTTACTTCTTT	33.3	25.0	54.8	52.7	175-205 bp	0.9	43.5
ycf1_p2	AGATTCTTTTGGTTTTGTGCAT	TCATACCAATCAATTTACTTCTTT	31.8	25.0	55.3	52.7	175-205 bp	0.9	43.5
ycf1_p3	GATTCTTTTGGTTTTGTGCA	TCATACCAATCAATTTACTTCTTT	35.0	25.0	53.1	52.7	174-204 bp	0.9	43.5
ycf1_p4	AGATTCTTTTGGTTTTGTGCA	CATACCAATCAATTTACTTCTTTT	33.3	25.0	54.8	52.0	174-204 bp	0.9	43.5
ycf1_p5	AAGATTCTTTTGGTTTTGTGCA	TCATACCAATCAATTTACTTCTTT	31.8	25.0	55.5	52.7	176-206 bp	0.9	43.4