Hotspot

ndhH-rps15

SSC · 127714-127874 · 161 bp

Back to Actaea

Pi 0.0427
MarkerSeek score 71.90
Primer available yes
Label ndhH-rps15

Feature Metadata

label_name
ndhH-rps15
feature_type
igs
region
SSC
length_bp
161
variable_sites
21
pi
0.0427
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.91
markerseek_score
71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH-rps15_p1 AACACCATGCATTGATGGGT TCGGGAGCTAAAAACTCGTT 45.0 45.0 58.1 57.2 230-259 bp 1.0 84.5
ndhH-rps15_p2 AACACCATGCATTGATGGGT TTCGGGAGCTAAAAACTCGT 45.0 45.0 58.1 57.2 231-260 bp 1.0 84.4
ndhH-rps15_p3 GAGTCGAAGAACACCATGCA TCGGGAGCTAAAAACTCGTT 50.0 45.0 58.2 57.2 239-268 bp 1.0 83.0
ndhH-rps15_p4 GAGTCGAAGAACACCATGCA TTCGGGAGCTAAAAACTCGT 50.0 45.0 58.2 57.2 240-269 bp 1.0 83.0
ndhH-rps15_p5 TGAGTCGAAGAACACCATGC TCGGGAGCTAAAAACTCGTT 50.0 45.0 58.2 57.2 240-269 bp 1.0 83.0