Hotspot

ndhG-ndhI

SSC · 123502-123900 · 399 bp

Back to Actaea

Pi 0.0277
MarkerSeek score 63.40
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
399
variable_sites
31
pi
0.0277
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.96
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 AACAGCCCAAAGACGGAAAT TTAGGACGGTTGCCAATGTC 45.0 50.0 58.0 58.2 797-813 bp 0.9 80.3
ndhG-ndhI_p2 AACAGCCCAAAGACGGAAAT GACGGTTGCCAATGTCAGTA 45.0 50.0 58.0 58.2 793-809 bp 0.9 80.1
ndhG-ndhI_p3 AACAGCCCAAAGACGGAAAT CCTATCCCATGCGAATCGTT 45.0 50.0 58.0 57.8 1134-1150 bp 0.9 79.0
ndhG-ndhI_p4 AACAGCCCAAAGACGGAAAT GGCTGCAAGGTATATCGGTC 45.0 55.0 58.0 58.2 1170-1186 bp 0.9 78.6
ndhG-ndhI_p5 AACAGCCCAAAGACGGAAAT AGTACGGGCTGCAAGGTATA 45.0 50.0 58.0 58.2 1176-1192 bp 0.9 78.6