Hotspot

ycf1-ndhF

SSC · 115366-115448 · 83 bp

Back to Actaea

Pi 0.221
MarkerSeek score 72.40
Primer available yes
Label ycf1-ndhF

Feature Metadata

label_name
ycf1-ndhF
feature_type
igs
region
SSC
length_bp
83
variable_sites
41
pi
0.221
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.90
markerseek_score
72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-ndhF_p1 ACGAAGGGGACTAAACAGGA TAAGGGGATTGGCGGAACTA 50.0 50.0 58.0 58.1 534-664 bp 0.9 86.9
ycf1-ndhF_p2 ACAGGAACAAGAGGGATCCA TAAGGGGATTGGCGGAACTA 50.0 50.0 58.0 58.1 520-650 bp 0.9 86.7
ycf1-ndhF_p3 AAGAGGGATCCACCGAAGAA TAAGGGGATTGGCGGAACTA 50.0 50.0 58.0 58.1 512-642 bp 0.9 86.7
ycf1-ndhF_p4 CCTTCCCCTTCCCTTTGTTC TAAGGGGATTGGCGGAACTA 55.0 50.0 58.1 58.1 489-619 bp 0.9 86.5
ycf1-ndhF_p5 TTTAGGCAGAATACCGTCGC TAAGGGGATTGGCGGAACTA 50.0 50.0 58.1 58.1 649-779 bp 0.9 85.3