Hotspot

ndhJ-ndhK

LSC · 53519-53666 · 148 bp

Pi 0.0316

MarkerSeek score 67.60

Primer available yes

Label ndhJ-ndhK

Feature Metadata

label_name: ndhJ-ndhK
feature_type: igs
region: LSC
length_bp: 148
variable_sites: 15
pi: 0.0316
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-ndhK_p1	CTAGCCAAGCAGACAAACGA	TCTTCCCCCGAATTAGTGAA	50.0	45.0	58.2	55.8	195-200 bp	1.0	69.6
ndhJ-ndhK_p2	CCTTGGTAATCGAAGCCCAA	TCTTCCCCCGAATTAGTGAA	50.0	45.0	57.9	55.8	241-246 bp	1.0	69.0
ndhJ-ndhK_p3	CCTTGGTAATCGAAGCCCAA	TCAGTATCTTCCCCCGAATT	50.0	45.0	57.9	55.6	247-252 bp	1.0	67.2
ndhJ-ndhK_p4	CCTTGGTAATCGAAGCCCAA	TTCAGTATCTTCCCCCGAAT	50.0	45.0	57.9	55.6	248-253 bp	1.0	67.2
ndhJ-ndhK_p5	CCTTGGTAATCGAAGCCCAA	ATCTTCCCCCGAATTAGTGA	50.0	45.0	57.9	55.6	242-247 bp	1.0	67.0