Hotspot

ycf3-trnS-GGA

LSC · 48222-49151 · 930 bp

Back to Actaea

Pi 0.0186
MarkerSeek score 71.40
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
930
variable_sites
49
pi
0.0186
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 CATTTATCCGCGATCTAGGCA CCCTCGGTAAACAAAAGCCT 47.6 50.0 58.0 58.1 988-1029 bp 1.0 78.6
ycf3-trnS-GGA_p2 CATTTATCCGCGATCTAGGCA ACGGAAAGAGAGGGATTCGA 47.6 50.0 58.0 58.2 1009-1050 bp 1.0 78.0
ycf3-trnS-GGA_p3 CATTTATCCGCGATCTAGGCA TTCCAATGCTACGCCTTGAA 47.6 45.0 58.0 57.8 959-1000 bp 1.0 78.0
ycf3-trnS-GGA_p4 CATTTATCCGCGATCTAGGCA GACGGAAAGAGAGGGATTCG 47.6 55.0 58.0 57.8 1010-1051 bp 1.0 77.5
ycf3-trnS-GGA_p5 CATTTATCCGCGATCTAGGCA ACCCTCGGTAAACAAAAGCC 47.6 50.0 58.0 58.4 989-1030 bp 1.0 76.2