Hotspot

trnD-GUC-trnY-GUA

LSC · 33258-33698 · 441 bp

Back to Actaea

Pi 0.0343
MarkerSeek score 74.90
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
441
variable_sites
39
pi
0.0343
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.96
markerseek_score
74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 GTGCTCTGACCGATTGAACT TGGCAATATGTCTACGCTGG 50.0 50.0 57.9 57.8 489-513 bp 1.0 87.3
trnD-GUC-trnY-GUA_p2 GTGCTCTGACCGATTGAACT GGCAATATGTCTACGCTGGT 50.0 50.0 57.9 57.8 488-512 bp 1.0 87.3
trnD-GUC-trnY-GUA_p3 AGTCATCGGGTACTAGGTCG TGGCAATATGTCTACGCTGG 55.0 50.0 58.0 57.8 553-577 bp 1.0 86.6
trnD-GUC-trnY-GUA_p4 AGTCATCGGGTACTAGGTCG GGCAATATGTCTACGCTGGT 55.0 50.0 58.0 57.8 552-576 bp 1.0 86.6
trnD-GUC-trnY-GUA_p5 AGTCATCGGGTACTAGGTCG GGGGACGGACTGTAAATTCG 55.0 55.0 58.0 58.4 574-598 bp 1.0 85.4