Hotspot

trnK-UUU-rps16

LSC · 4365-5141 · 777 bp

Back to Actaea

Pi 0.0283
MarkerSeek score 73.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
777
variable_sites
60
pi
0.0283
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT CGCATTAATATTAATTACACGAAA 50.0 25.0 58.2 52.5 838-843 bp 0.2 8.5
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT CGCATTAATATTAATTACACGAAA 50.0 25.0 58.2 52.5 837-842 bp 0.2 8.5
trnK-UUU-rps16_p3 TCTAGCCGCACTTAAAAGCC CGCATTAATATTAATTACACGAAA 50.0 25.0 58.3 52.5 852-857 bp 0.2 8.5
trnK-UUU-rps16_p4 CCGCACTTAAAAGCCGAGTA CGCATTAATATTAATTACACGAAA 50.0 25.0 58.3 52.5 847-852 bp 0.2 8.5
trnK-UUU-rps16_p5 TACCGTTGAGTTAGCAACCC CGCATTAATATTAATTACACGAAA 50.0 25.0 57.5 52.5 824-829 bp 0.2 8.5