Hotspot

trnH-GUG-psbA

LSC · 120-438 · 319 bp

Back to Actaea

Pi 0.0546
MarkerSeek score 85.40
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
319
variable_sites
46
pi
0.0546
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.97
markerseek_score
85.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG GCGCTAACCTTGGTATGGAA 55.0 50.0 58.1 58.0 420-465 bp 1.0 90.7
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 434-479 bp 1.0 90.7
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 433-478 bp 1.0 90.7
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 435-480 bp 1.0 90.7
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG TATCATCAACCGCGCTAACC 55.0 50.0 58.1 58.1 431-476 bp 1.0 89.9