Hotspot

ycf1

SSC · 126215-131692 · 5478 bp

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Pi 0.00803

MarkerSeek score 43.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5478
variable_sites: 44
pi: 0.00803
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTGTGACATTTCATTTTTAACAGCA	TCGCTTTAAAGAGACATGCGA	32.0	42.9	57.5	58.0	241 bp	1.0	42.0
ycf1_p2	GTGTGACATTTCATTTTTAACAGCA	AGCATGAATTCCACTTTCGC	32.0	45.0	57.5	57.1	257 bp	1.0	41.9
ycf1_p3	GTGTGACATTTCATTTTTAACAGCA	GCATGAATTCCACTTTCGCT	32.0	45.0	57.5	57.1	256 bp	1.0	41.9
ycf1_p4	AGTGTGACATTTCATTTTTAACAGC	AGCATGAATTCCACTTTCGC	32.0	45.0	57.3	57.1	258 bp	1.0	41.9
ycf1_p5	AGTGTGACATTTCATTTTTAACAGC	GCATGAATTCCACTTTCGCT	32.0	45.0	57.3	57.1	257 bp	1.0	41.9