Hotspot

rps14

LSC · 38572-38874 · 303 bp

Back to Acronychia

Pi 0.0033

MarkerSeek score 43.00

Primer available yes

Label rps14

Feature Metadata

label_name: rps14
feature_type: gene
region: LSC
length_bp: 303
variable_sites: 1
pi: 0.0033
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14_p1	TACCCCGCGATCAAAAGAAG	TTCTGTCTTGTATCCGTGCG	50.0	50.0	58.0	58.0	610-625 bp	1.0	82.5
rps14_p2	TACCCCGCGATCAAAAGAAG	GGCATTGGAAACACATCACG	50.0	50.0	58.0	58.0	1039-1054 bp	1.0	81.4
rps14_p3	GAATGCGCCCCTTTACCATA	TTCTGTCTTGTATCCGTGCG	50.0	50.0	58.0	58.0	561-563 bp	1.0	80.7
rps14_p4	CTACCCCGCGATCAAAAGAA	TTCTGTCTTGTATCCGTGCG	50.0	50.0	58.0	58.0	626 bp	0.5	59.8
rps14_p5	CTACCCCGCGATCAAAAGAA	GGCATTGGAAACACATCACG	50.0	50.0	58.0	58.0	1055 bp	0.5	59.7