Hotspot

petD

LSC · 78309-79503 · 1195 bp

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Pi 0.0109

MarkerSeek score 57.40

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1195
variable_sites: 13
pi: 0.0109
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	GGAAATTTCCCCTCAACTCCA	TCCCTAGATACATACGTCGTGA	47.6	45.5	57.8	57.7	1325 bp	1.0	52.7
petD_p2	GGAAATTTCCCCTCAACTCCA	ACTCCCTAGATACATACGTCGT	47.6	45.5	57.8	57.7	1327 bp	1.0	52.6
petD_p3	GGAAATTTCCCCTCAACTCCA	TGACTTTGAAGTGACTACTCCC	47.6	45.5	57.8	57.4	1343 bp	1.0	50.6
petD_p4	GGAAATTTCCCCTCAACTCCA	CTCCCTAGATACATACGTCGTG	47.6	50.0	57.8	57.3	1326 bp	1.0	50.1
petD_p5	GGAAATTTCCCCTCAACTCCA	CCCTAGATACATACGTCGTGA	47.6	47.6	57.8	56.2	1324 bp	1.0	49.0