Hotspot

rpl32-trnL-UAG

SSC · 115387-116378 · 992 bp

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Pi 0.0121
MarkerSeek score 54.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
992
variable_sites
12
pi
0.0121
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGGGAGGGTATTGGTCAACA AGCGTGTCTACCGATTTCAC 50.0 50.0 57.9 58.0 1131-1135 bp 1.0 80.4
rpl32-trnL-UAG_p2 AGGGAGGGTATTGGTCAACA CAGCGTGTCTACCGATTTCA 50.0 50.0 57.9 58.0 1132-1136 bp 1.0 80.3
rpl32-trnL-UAG_p3 AGGGAGGGTATTGGTCAACA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.9 57.9 1141-1145 bp 1.0 79.4
rpl32-trnL-UAG_p4 AGGGAGGGTATTGGTCAACA CCTAAGAGCAGCGTGTCTAC 50.0 55.0 57.9 57.8 1140-1144 bp 1.0 78.8
rpl32-trnL-UAG_p5 AGGGAGGGTATTGGTCAACA CTAAGAGCAGCGTGTCTACC 50.0 55.0 57.9 57.8 1139-1143 bp 1.0 78.8