Hotspot

atpB-rbcL

LSC · 55953-56717 · 765 bp

Back to Acronychia

Pi 0.00523

MarkerSeek score 61.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 765
variable_sites: 4
pi: 0.00523
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ACCGGACCAATGATTTGAGC	GCTTTGAATCCAACACTCGC	50.0	50.0	58.5	58.0	898-900 bp	1.0	75.8
atpB-rbcL_p2	GGCCGGGAGTAGTAGGATTT	GCTTTGAATCCAACACTCGC	55.0	50.0	58.6	58.0	837-839 bp	1.0	75.5
atpB-rbcL_p3	CGGACCAATGATTTGAGCGA	GCTTTGAATCCAACACTCGC	50.0	50.0	58.6	58.0	896-898 bp	1.0	75.1
atpB-rbcL_p4	ACCGGACCAATGATTTGAGC	AACACCGGCTTTGAATCCAA	50.0	45.0	58.5	58.3	905-907 bp	1.0	73.7
atpB-rbcL_p5	GGCCGGGAGTAGTAGGATTT	AACACCGGCTTTGAATCCAA	55.0	45.0	58.6	58.3	844-846 bp	1.0	73.3