Hotspot

trnT-GGU-psbD

LSC · 32972-34233 · 1262 bp

Back to Acronychia

Pi 0.00476

MarkerSeek score 61.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1262
variable_sites: 6
pi: 0.00476
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	AAAAACGAATCGGTCCCTCC	55.0	50.0	58.1	58.2	1410-1425 bp	1.0	78.5
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	AAAAACGAATCGGTCCCTCC	50.0	50.0	58.1	58.2	1413-1428 bp	1.0	78.3
trnT-GGU-psbD_p3	TCCGTCTAGTGACTCCTTCC	AAAAACGAATCGGTCCCTCC	55.0	50.0	57.9	58.2	1460-1475 bp	1.0	78.0
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	GACCGGACCAACCTACAAAA	55.0	50.0	58.1	57.7	1426-1441 bp	1.0	77.9
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	ACAAAAACGAATCGGTCCCT	55.0	45.0	58.1	57.7	1412-1427 bp	1.0	77.8