Hotspot

trnS-GCU-trnG-UCC

LSC · 8188-9148 · 961 bp

Back to Acronychia

Pi 0.00628

MarkerSeek score 49.90

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 961
variable_sites: 6
pi: 0.00628
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CCGACGCTTTAATCCACTCA	TACCACTAAACTATACCCGC	50.0	45.0	58.0	54.0	1006-1012 bp	1.0	48.8
trnS-GCU-trnG-UCC_p2	CCGACGCTTTAATCCACTCA	TTACCACTAAACTATACCCGC	50.0	42.9	58.0	54.9	1007-1013 bp	1.0	47.7
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	TACCACTAAACTATACCCGC	50.0	45.0	58.2	54.0	1064-1070 bp	1.0	47.6
trnS-GCU-trnG-UCC_p4	ACGCTTTAATCCACTCAGCC	TACCACTAAACTATACCCGC	50.0	45.0	58.3	54.0	1003-1009 bp	1.0	46.8
trnS-GCU-trnG-UCC_p5	CGCTTTAATCCACTCAGCCA	TACCACTAAACTATACCCGC	50.0	45.0	58.3	54.0	1002-1008 bp	1.0	46.8