Hotspot

trnH-GUG-psbA

LSC · 88-440 · 353 bp

Back to Acronychia

Pi 0.0567

MarkerSeek score 83.20

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 353
variable_sites: 20
pi: 0.0567
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	CGTGCTAACCTTGGTATGGA	50.0	50.0	57.9	57.3	478-479 bp	1.0	76.8
trnH-GUG-psbA_p2	CTGCCTTAATCCACTTGGCT	TCGTGCTAACCTTGGTATGG	50.0	50.0	57.9	57.3	479-480 bp	1.0	76.8
trnH-GUG-psbA_p3	ACTGCCTTAATCCACTTGGC	CGTGCTAACCTTGGTATGGA	50.0	50.0	58.2	57.3	479-480 bp	1.0	76.6
trnH-GUG-psbA_p4	ACTGCCTTAATCCACTTGGC	TCGTGCTAACCTTGGTATGG	50.0	50.0	58.2	57.3	480-481 bp	1.0	76.6
trnH-GUG-psbA_p5	CAACCCACTGCCTTAATCCA	CGTGCTAACCTTGGTATGGA	50.0	50.0	57.8	57.3	485-486 bp	1.0	76.1