Hotspot

trnL-CAA-ycf2

IRa · 145528-146193 · 666 bp

Back to Acronema

Pi 0.0957

MarkerSeek score 83.20

Primer available yes

Label trnL-CAA-ycf2

Feature Metadata

label_name: trnL-CAA-ycf2
feature_type: igs
region: IRa
length_bp: 666
variable_sites: 154
pi: 0.0957
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf2_p1	AAATGGTAGACACGCGAGAC	CAGCCCTTTGTCTCTGTGTT	50.0	50.0	58.0	58.0	591-1167 bp	0.588235	70.4
trnL-CAA-ycf2_p2	TGAAATGGTAGACACGCGAG	CAGCCCTTTGTCTCTGTGTT	50.0	50.0	58.0	58.0	593-1169 bp	0.588235	70.4
trnL-CAA-ycf2_p3	AAATGGTAGACACGCGAGAC	GTTCTTGCAGAGTGGAACCA	50.0	50.0	58.0	58.0	715-1291 bp	0.588235	70.2
trnL-CAA-ycf2_p4	AAATGGTAGACACGCGAGAC	GGCAAGCGGATCCTTTATGA	50.0	50.0	58.0	58.0	768-1344 bp	0.588235	70.0
trnL-CAA-ycf2_p5	AAATGGTAGACACGCGAGAC	TAGCCGGAAAGTTATGTGGC	50.0	50.0	58.0	58.0	760-797 bp	0.529412	66.8