Hotspot

ndhF-rpl32

SSC · 113090-114214 · 1125 bp

Back to Acronema

Pi 0.0544

MarkerSeek score 75.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1125
variable_sites: 236
pi: 0.0544
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	AAGCGGGTCGATCAAGTAAC	ATTCCAGGCTTTTGTCGGTT	50.0	45.0	58.0	58.0	1286-2156 bp	0.764706	82.5
ndhF-rpl32_p2	CCGTGTGAACCAAAACCCTA	ATTCCAGGCTTTTGTCGGTT	50.0	45.0	58.0	58.0	1514-2384 bp	0.764706	82.3
ndhF-rpl32_p3	AAGCGGGTCGATCAAGTAAC	AATTCCAGGCTTTTGTCGGT	50.0	45.0	58.0	58.0	1287-2157 bp	0.705882	80.2
ndhF-rpl32_p4	CCGTGTGAACCAAAACCCTA	AATTCCAGGCTTTTGTCGGT	50.0	45.0	58.0	58.0	1515-2385 bp	0.705882	79.9
ndhF-rpl32_p5	GTTGCCGACGGAAAAAGAAG	ATTCCAGGCTTTTGTCGGTT	50.0	45.0	58.0	58.0	1108-1978 bp	0.705882	79.9