Hotspot

ycf2-trnL-CAA

IRb · 93192-93857 · 666 bp

Back to Acronema

Pi 0.0925

MarkerSeek score 83.20

Primer available yes

Label ycf2-trnL-CAA

Feature Metadata

label_name: ycf2-trnL-CAA
feature_type: igs
region: IRb
length_bp: 666
variable_sites: 146
pi: 0.0925
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnL-CAA_p1	CAGCCCTTTGTCTCTGTGTT	AAATGGTAGACACGCGAGAC	50.0	50.0	58.0	58.0	591-1167 bp	0.588235	70.4
ycf2-trnL-CAA_p2	CAGCCCTTTGTCTCTGTGTT	TGAAATGGTAGACACGCGAG	50.0	50.0	58.0	58.0	593-1169 bp	0.588235	70.4
ycf2-trnL-CAA_p3	GTTCTTGCAGAGTGGAACCA	AAATGGTAGACACGCGAGAC	50.0	50.0	58.0	58.0	715-1291 bp	0.588235	70.2
ycf2-trnL-CAA_p4	GGCAAGCGGATCCTTTATGA	AAATGGTAGACACGCGAGAC	50.0	50.0	58.0	58.0	768-1344 bp	0.588235	70.0
ycf2-trnL-CAA_p5	TAGCCGGAAAGTTATGTGGC	AAATGGTAGACACGCGAGAC	50.0	50.0	58.0	58.0	760-797 bp	0.529412	67.4