Hotspot

petA-psbJ

LSC · 63722-64692 · 971 bp

Back to Acronema

Pi 0.0422

MarkerSeek score 77.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 971
variable_sites: 176
pi: 0.0422
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CAGGGCCAGAACTTCTTGTT	AACTGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	1605-1804 bp	1.0	91.9
petA-psbJ_p2	CAGGGCCAGAACTTCTTGTT	ACTGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1604-1803 bp	1.0	91.9
petA-psbJ_p3	CGGATTTGGTCAGGGAGATG	AACTGGCCGTTTTGATCCTT	55.0	45.0	58.0	58.0	1529-1728 bp	0.882353	86.9
petA-psbJ_p4	CGGATTTGGTCAGGGAGATG	ACTGGCCGTTTTGATCCTTT	55.0	45.0	58.0	58.0	1528-1727 bp	0.882353	86.9
petA-psbJ_p5	CAGGGCCAGAACTTCTTGTT	TGCGGGTTGGTTATTTGTCA	50.0	45.0	58.0	58.0	1884 bp	0.117647	44.5